primary human mammary epithelial cell hmec culture hmecs  (ATCC)

Journal: The Journal of Biological Chemistry

Article Title: Clostridioides difficile TcdB induces expression of its receptor (CSPG4) through a noncanonical Hippo signaling mechanism

doi: 10.1016/j.jbc.2026.111137

Figure Lengend Snippet: Core Hippo kinase regulation of CSPG4 in TcdB exposed pericytes . A , pericytes were exposed to 1 ng/ml of TcdB2 for 24 h and colonic epithelial cells were exposed to 10 ng/ml of TcdB2 for 24 h. Multiple wells from each treatment were combined for RNA isolation. RT-qPCR was used to quantify CSPG4 transcripts. RT-qPCR data are presented as mean (n = 3) ± S.D. with each data point as a technical replicate. ∗ p < 0.05 determined by Student’s t test. B , Immunoblots from protein lysates acquired from pericytes exposed for 24 h to 1 ng/ml of TcdB. C , immunoblots from protein lysates obtained from pericytes or HeLa cells exposed for 24 h to 1 ng/ml of TcdB. Phosphorylated LATS1 (pLATS1) was detected with an antibody recognizing phosphorylation at Thr 1079. D , densitometry analysis of pLATS1 immunoblots. Relative band density is presented as mean (n = 2) ± S.D. with each data point as a technical replicate. ∗ p < 0.05 determined by Student’s t test. E , immunoblots from protein lysates acquired from pericyte or HeLa cells exposed for 24 h to 1 ng/ml of TcdB in the presence or absence of 10 μM XMU-MP-1. F , immunoblots from protein lysates taken from HeLa cells exposed for 24 h to 1 ng/ml of TcdB with and without of 30 μM TRULI.

Article Snippet: Primary human colonic epithelial cells (36,037–08) were obtained from Celprogen and were cultured using the protocol provided by Celprogen.

Techniques: Isolation, Quantitative RT-PCR, Western Blot, Phospho-proteomics

Journal: Antibiotics

Article Title: Activity of Natural Substances and n-Undecyl-α/β- l -Fucopyranoside Against the Formation of Pathogenic Biofilms by Pseudomonas aeruginosa

doi: 10.3390/antibiotics15010076

Figure Lengend Snippet: Influence of culture media and low molecular substances on the biofilm growth of P. aeruginosa ATCC 27853. Biofilms were determined via counting CFUs; 1 cm latex catheter pieces served as substrate for biofilm growth. The culture medium for tests was AU, and the incubation time was 24 h at 37 °C. ( a ) Comparing two different media; left: artificial urine (AU), right: Lysogeny Broth Medium (LBM). For statistical analysis a two-tailed t -test was used instead of an ANOVA analysis. ( b ) 512 µg/mL curcumin (CURC) and 5120 µg/mL Soluplus ® (SOL), ( c ) 256 µg/mL 1-monolaurin (ML) and 48 µg/mL Prontosan ® (PRT), and ( d ) 512 µg/mL 1-monolaurin (ML) and 5120 µg/mL Soluplus ® (SOL). ** for p ≤ 0.01; *** p ≤ 0.001 and **** p < 0.0001 indicates the degree of significance.; ns indicates no significance.

Article Snippet: Finally, 100 μL of the P. aeruginosa ATCC 27853 primary culture, diluted with sterile PBS buffer to an optical density (OD λ=600 nm ) of 0.02, was added.

Techniques: Incubation, Two Tailed Test

Journal: Antibiotics

Article Title: Activity of Natural Substances and n-Undecyl-α/β- l -Fucopyranoside Against the Formation of Pathogenic Biofilms by Pseudomonas aeruginosa

doi: 10.3390/antibiotics15010076

Figure Lengend Snippet: n-Undecyl-α/β- l -fucopyranoside, an inhibitor of the biofilm growth of P. aeruginosa ATCC 27853. Biofilms were determined via counting CFUs; 1 cm latex catheter pieces served as the substrate for biofilm growth. The culture medium for tests was AU, and the incubation time was 24 h at 37 °C. ( a ) n-Undecyl-α/β- l -fucopyranoside (nU-Fuc) in different amounts, µg/mL; *** for p ≤ 0.001. ( b ) n-Undecyl-α/β- l -fucopyranoside (nU-Fuc) in 32–512 µg/mL as inhibitor of biofilm growth of P. aeruginosa PA 01; ** for p ≤ 0.01. The p -value indicates the degree of significance; ns indicates no significance.

Article Snippet: Finally, 100 μL of the P. aeruginosa ATCC 27853 primary culture, diluted with sterile PBS buffer to an optical density (OD λ=600 nm ) of 0.02, was added.

Techniques: Incubation

Journal: Antibiotics

Article Title: Activity of Natural Substances and n-Undecyl-α/β- l -Fucopyranoside Against the Formation of Pathogenic Biofilms by Pseudomonas aeruginosa

doi: 10.3390/antibiotics15010076

Figure Lengend Snippet: Influence of terrein on the biofilm growth of P. aeruginosa ATCC 27853. Biofilms were determined via counting CFUs. 1 cm latex catheter pieces served as the substrate for biofilm growth. The culture medium for tests was AU, and the incubation time was 24 h at 37 °C. ( a ) Terrein (TER) in different concentrations, µg/mL; *** for p < 0.001, **** p < 0.0001. ( b ) 256 µg/mL terrein and 64 µg/mL Prontosan ® (PRT); * for p < 0.05. ( c ) Molecular structure of terrein The p -value indicates the degree of significance; ns indicates no significance.

Article Snippet: Finally, 100 μL of the P. aeruginosa ATCC 27853 primary culture, diluted with sterile PBS buffer to an optical density (OD λ=600 nm ) of 0.02, was added.

Techniques: Incubation

Journal: Antibiotics

Article Title: Activity of Natural Substances and n-Undecyl-α/β- l -Fucopyranoside Against the Formation of Pathogenic Biofilms by Pseudomonas aeruginosa

doi: 10.3390/antibiotics15010076

Figure Lengend Snippet: Experimental setup. ( a ) −80 °C glycerol stock of P. aeruginosa ATCC 27853, ( b ) the spread on LB agar with a following 16 h incubation, ( c ) primary culture of P. aeruginosa ATCC 27853, ( d ) secondary culture with three catheter pieces in medium, ( e ) ten-fold dilution series made from biofilm suspension obtained from processed catheter-pieces, and ( f ) the spread appropriate dilutions on LB agar with following 24 h incubation for CFU determination.

Article Snippet: Finally, 100 μL of the P. aeruginosa ATCC 27853 primary culture, diluted with sterile PBS buffer to an optical density (OD λ=600 nm ) of 0.02, was added.

Techniques: Incubation, Suspension